<i>B. cinerea</i> induced <i>ACS2</i> and <i>ACS6</i> gene activation is dependent on functional MPK3 and MPK6.

<p>Twelve-day-old wild type (Col-0), <i>mpk3</i>, <i>mpk6</i>, and rescued <i>mpk3/mpk6</i> double mutant seedlings grown in GC vials were inoculated with <i>B. cinerea</i> spores. Samples were collected at indicated times. Total RNAs were extracted and treated with DNase to remove trace genomic DNA contamination. After reverse transcription, expressions of <i>ACS2</i> (A) and <i>ACS6</i> (B) genes were quantified by real-time PCR. <i>ACS</i> transcript levels were calculated as a percentage of the <i>EF1α</i> transcript. Error bars indicate standard deviations (n = 3).