IFNβ inhibits IL-1β release in the presence of activated human CD4+CD45RO+ memory T-cells.

<p>Monocytes were incubated overnight in the absence or presence of unstimulated or soluble αCD3 (0.5 ug/ml) activated CD4+CD45RO+CD45RA- memory T-cells with or without 1000 IU/ml IFNβ. The next day 100 ng/ml LPS was added to the co-culture for 4 h, followed by addition of 500 uM ATP for 45 min; a) IL-1β (n = 8) and b) TNFα levels (n = 5) were measured in the supernatant by ELISA. There was a significant reduction of IL-1β release in the presence of activated T-cells and IFNβ; TNFα secretion was not affected; c) memory T-cells were incubated with IFNβ either in the presence or absence of 2 ug/ml αCD3 and αCD28 for 18 h, washed twice with PBS and co-incubated with monocytes for 14 h. The release of IL-1β by monocytes after stimulation with LPS and ATP was suppressed by co-incubation with activated IFNβ-primed memory T-cells (n = 3) (Data are shown as means ± SD of duplicate cultures; *p<0.05; ***p<0.001 employing repeated measures ANOVA with post-hoc Bonferroni adjustment for multiple comparisons to avoid random correlations); d) immunoblot of pro-IL-1β cleavage and pro-caspase-1 levels in the cell lysate of monocytes.