PI(3,4,5)P and PI(4,5)P are generated during CEACAM1- and 3- mediated bacterial entry.

<p>(<b>A</b>) 293 cells were cotransfected with constructs encoding mKate-tagged CEACAM1-4L or CEACAM3 together with GFP-tagged Btk-PH domain (which binds specifically to PI(3,4,5)P). Cells were left uninfected or were infected with Pacific Blue labelled Opa_CEA-expressing <i>N. gonorrhoeae</i> for 60 min, fixed and analysed by confocal microscopy. Arrowheads highlight bacteria associated with CEACAMs and the Btk-PH domain. Insets show enlargement of the highlighted area. Bars represent 5 µm. (<b>B</b>) Cells were cotransfected with CEACAM constructs as in A) together with the GFP-tagged PLC-δ-PH domain (which specifically binds to PI(4,5)P). Cells were infected and analyzed as in (A). Arrowheads highlight bacteria associated with CEACAMs and the PLC-δ-PH domain. Insets show enlargement of the highlighted area. Bars represent 5 µm.