Effect of chemerin on the production of cytokines by activated mouse macrophages.

<p>Peritoneal macrophages from WT mice, collected after i.p. thioglycolate injection, were exposed or not for 1 h to 100 nM recombinant mouse chemerin. The cells were further stimulated by 100 ng/ml LPS. Unstimulated cells were used as controls. (A) At the indicated time points, cells were harvested for the determination of transcript levels for IL-6, TNF-α and IL-10, using quantitative RT-PCR. Results are expressed as mean ± SEM for 2 independent experiments performed in duplicate. (B) At the indicated time points, IL-6, TNF-α and IL-10 levels were determined in the supernatants by ELISA. Results are expressed as mean ± SEM for 3 independent experiments performed in duplicate.