Mis-expression of MSL2-YFP V273S, L277S or V273S/L277S causes severe defects in leaf growth and chloroplast integrity.

<p>(A) Number of T1 plants recovered from side-by-side plant transformations and phenotypes of subsequent T2 lines. (B) Representative images of 2 week-old T2 seedlings. Size bar is 4 mm. (C) Top, representative confocal scanning images of cotyledons of the indicated T2 seedlings. Chlorophyll autofluorescence is pseudocolored red. Size bar is 30 microns. Bottom, representative T2 seedlings stained with Lugol's reagent to indicate starch content. Scale bar is 1 mm. Note the loss of all leaf primordial, as frequently observed in these lines. (D) Agarose gel of PCR products specific to the transgenes and genes indicated to the right. cDNA derived from the indicated T2 lines shown in (D) was used as template.