MCM2 (FL and mutants) interacts with PP2A.

<p>(<b>A</b>) The <i>Mcm2-FL</i>- or mutant-transfected 3T3 cells (left) and <i>gp70</i>/<i>Mcm2-FL-</i> or <i>gp70</i>/mutants-transfected 3T3 cells (right) were treated with 1 µM doxorubicin for 24 h. Cell lysates were subjected to a pull-down assay to detect the binding of MCM2-FL or the mutants to PP2A. (<b>B</b>) 3T3 cells were pre-incubated with 10 nM okadaic acid (OA) and 10 µM NU7026 for 2 h, and treated with 1 µM doxorubicin for 24 h. The apoptotic cell ratio was determined with annexin V-staining. Asterisk (*) indicates <i>p</i><0.01 for control vs. mutant-transfected cells. Data represent the mean and 95% CI of 3 independent experiments. (<b>C</b>) Western blot analysis of 3T3 cells to detect phospho-DNA-PK. Note the significantly increased levels of DNA-PK-p2053 in OA-treated 3T3 cells, and the complete abrogation by NU7026.