Analysis of MHC class I cell surface expression in <i>lmp7</i><i>⁻</i>^{<b><i>/</i></b><i>−</i>} <b>Mefs expressing proLMP7- or proβ5-containing subunits.</b>

<p><i>lmp7^−/−</i> Mefs expressing LMP7-Flag, proLMP7mβ5-Flag, β5-Flag, proβ5mLMP7-Flag or cells transduced with the empty vector construct were cultured in the presence of 50 U/ml IFNγ for 4 days. WT-Mefs stimulated with IFNγ for 4 days were used as a positive control. The MHC class I cell surface expression of the haplotypes H-2K^b (E) and H-2D^b (F) on the various cell lines was determined by flow cytometry. The relative MHC class I density of the different cell lines was calculated by normalization of the median fluorescence intensity (MFI) of the indicated cell line to the MFI of <i>lmp7^−/−</i> Mefs transduced with the empty vector construct. Given values are means ± standard deviations of five independent experiments.