Ferritin reverses the anti-adhesive properties of HKa.

<p>Cells were incubated with HKa (50 nM) alone, HKa (50 nM) plus ferritin (100 nM), or ferritin (100 nM) alone and allowed to adhere on vitronectin-coated coverslips in the presence of 20 ng/ml bFGF and 10 µM ZnCl₂ for two hours. <b>A.</b> Adherent cells were fixed and stained with crystal violet and visualized by microscopy; <b>B.</b> Adherent cells per 10× field were counted; shown are means and standard deviation of 3 experiments with * p<0.001 and ** p<0.0001. <b>C.</b> Adherent cells were lysed and analyzed by western blotting. <b>D.</b> Phosphorylation of signaling molecules was quantified by densitometry. Data is expressed as percent phosphorylated/total protein; shown are means and standard deviation of three independent experiments; *p<0.02; **p<0.003.