Cloning and expression of novel Nesprin KASH and CH isoforms.

<p>A) Schematic representation of p53KASH^Nesp1 (Accession numberJQ754366) and p56CH^Nesp1 (Accession number JQ740783) relative to the nesprin-1 giant. B) p53KASH^Nesp1 localizes to the NE when transfected into U2OS cells. C) Nesprin-1 Flag-p56CH^Nesp1 localized to the nucleolus when transfected into U2OS cells. D) Nesprin-1 Flag-p56CH^Nesp1 localizes to actin stress fibres and with Focal Adhesion Kinase (FAK) at focal adhesions when transfected into Human Dermal Fibroblasts (HDFs). E) Nesprin-2 Flag-p32CH^Nesp2 (Accession numberJQ754367) co-localized with FAK at focal adhesions when transfected into U2OS cells. F) p53KASH^Nesp1 expression was not detected by PCR in U2OS, Human Dermal Fibroblasts (HDFs), Vascular Smooth Muscle Cells (VSMCs) or Myoblasts (MBs), however it was detected in the heart, spleen and peripheral blood leukocytes (PBL). p56CH^Nesp1 was detected in all cells and tissues examined whereas p32CH^Nesp2 was limited to U2OS cells, MBs and PBL.