Lack of flagellin reduces <i>P. aeruginosa</i> supernatant-induced mucin expression in human primary bronchial epithelial cells.

<p>Human primary bronchial epithelial cells were exposed for 24 hours to supernatants from either PAK or ΔFliC at 1∶8 dilution. Total mRNA was prepared and subjected to RT-qPCR to measure MUC5AC (A), MUC2 (B) and MUC5B (C). (D) Shows the amount of IL-8 secreted into culture supernatants of human primary bronchial epithelial following stimulation with either PAK or ΔFliC supernatant, as detected by ELISA. The Values represent means ± SM of 3 independent experiments from three different donors done in replicates. ns = no significant, *<i>P</i><0.05, **<i>P</i><0,01, ***<i>P</i><0,001 stimulated <i>vs.</i> unstimulated control cells.