UNC-89 is required for sarcomere organization and function.

<p>(A–C) Representative L1 animals labeled with an anti-myosin heavy chain-A (MHC-A) antibody. Scale bar = 2 µm. Anterior is up. (A) Wild-type animal showing normal MHC-A organization. (B, C) <i>unc-89</i> mutants indicating normal distribution of MHC-A. (D–F) Representative adult animals labeled with anti-MHC-A antibody. Scale bar = 5 µm. Anterior is to the left. (D) Wild-type animal showing normal MHC-A organization. (E, F) <i>unc-89</i> mutants have severely disorganized sarcomeres. Representative (G) wild-type and (H) <i>unc-89(ak155)</i> adult animals displaying normal sinusoidal locomotion. Scale bar = 500 µm. (J) Body bend analysis of animals crawling. <i>egl-19(n582); unc-89(ak155)</i> display a similar rate of body bends as compared to <i>egl-19(n582)</i> single mutants. However, <i>unc-89(ak155)</i>; <i>unc-22(e66)</i> and <i>egl-19(n582)unc-22(RNAi)</i> are completely paralyzed and do not produce any body bends, which is significantly worse than the single mutants (* p<0.05). (K) Body bend analysis of animals swimming. <i>unc-89(ak155); unc-68(r1162)</i> mutants show a similar rate of body bends as <i>unc-68(r1162)</i> single mutants (p = 0.48). However, <i>unc-89(ak155); unc-22(e66)</i> double mutants show a significant decrease in body bends compared to <i>unc-22(e66)</i> mutants alone (p<0.001).