TDP-1 expression is induced by proteotoxicity.

<p>(A–G) are low-resolution photographs of young adult transgenic worms. The images have been converted to black and white and photo-reversed to aid visualization. Difficult to see worms are outlined. (A) Low expression and nuclear localization of TDP-1::GFP under normal growth conditions. (B) The ER stressor tunicamycin highly induced TDP-1::GFP expression. (C) Wild type TDP-43 did not induce TDP-1::GFP expression in a <i>TDP-1::GFP</i>;<i>TDP-43 WT</i> transgenic strain. (D) Mutant TDP-43 induced TDP-1::GFP expression in a <i>TDP-1::GFP;TDP-43[A315T]</i> transgenic strain. (E) Wild type FUS did not induce TDP-1::GFP expression in a <i>TDP-1::GFP</i>;<i>FUS WT</i> transgenic strain. (F) Mutant FUS induced TDP-1::GFP expression in a <i>TDP-1::GFP;FUS[S57Δ]</i> transgenic strain. (G) Mutation in <i>daf-16</i> blocked the induction of TDP-1::GFP expression by tunicamycin treatment. Please also see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002806#pgen.1002806.s004" target="_blank">Figure S4</a>.