Western blots showing p21^waf1 expression and active Rho in TEC and TL cell lines.

<p>A(i) p21^waf1 protein in T-cell lymphoma and carcinoma cell lines, (ii) Plot showing the relative intensity of p21^waf1 protein in T-cell lymphoma and carcinoma cell lines relative to actin. Means and standard error of mean were determined from at least five independent experiments. (*p<0.05, **P<0.01, ***P<0.001, ****P<.0001). (B) <b>Measurement of active RhoGTP in cell lines</b>. (i) RhoGTP pull down assay showing constitutive and LPA induced levels of active RhoGTP in T-cell lymphoma and thymic epithelial carcinoma cell lines. (ii) Plot showing the RhoGTP to total Rho protein ratio by scanning the relative intensity of the protein bands in non-treated and LPA induced in TEC, TL, MEF cell lines. Means and standard error of mean determined from four independent experiments. (*p<0.05, **P<0.01, ***P<0.001 and ****P<.0001 by Student t test and two way ANOVA test.