Microtubule disorganization and retraction but not degeneration take place during NMJ remodeling. A-F

<p>, Anti-Acetylated Tubulin (red) and anti-HRP (green) staining at 2 h, 5 h and 7 h APF. Tubulin staining was present and continuous at the NMJ in all the stages analyzed, and was absent in the HRP-labeled debris (arrowhead in F). <b>G-L</b>, Anti-Futsch (red) and anti-HRP (green) staining. The insets correspond to high magnifications. (I, J) 5 h APF, the presynaptic microtubules become thicker. (K, L) 7 h APF, the thickness of the microtubule bundle is maintained. Futsch was absent in HRP-labelled debris (arrowhead in L). Bars, 20 µm. <b>M</b>, Diameter of the distal end of the axons at 2 h (n = 17), 5 h (n = 16) and 7 h (n = 18) APF. Results are mean and s.e.m. ***: P<0.001, ns: no statistical difference (t-test). Genotype: <i>MHC-mGFP-Shaker.</i>