Glia invade NMJ early in metamorphosis and dynamically retracts before motoneuron pruning. A-H,

<p>Glial extensions were observed (green) at 2 h APF by expressing <i>UAS-mCD8-GFP</i> with the <i>repo-GAL4</i> driver. The NMJ was also labeled with HRP (blue) and DLG (red). (A-D) A blunt-ended glial process is seen in A. A lamellipodium process is shown in E. Additional merges including the anti-DLG staining are shown in D and H. Here, the arrowheads point to the end of the glial extension. Bars, 30 µm. <b>I</b>, Glial projections were scored at 2 h (n = 17), 5 h (n = 20) and 7 h (n = 26) APF. The percentages of lamellipodia, blunt-ended and no glial extensions are represented. *: P<0.05, ***: P<0.001 (χ² test). Genotype: <i>2× UAS-mGFP/+</i>; <i>repo-GAL4/+.</i>