Intranasal SEA challenge induces cellular infiltration, protein leakage and inflammation.

<p>Mice received 1 <i>µ</i>g of SEA or vehicle alone i.n., secondary (2°) challenge of either vehicle or SEA 48 h after the primary (1°) and were sacrificed 5 h later (53 hrs total). (A) Cells present in BAL fluid were harvested and enumerated and (B) total BAL fluid protein quantified by BCA. (C) IFNγ in BAL fluid and (D) in serum, and (E) IL-6 in BAL and (F) in serum were quantified by ELISA. (G) LDH in BAL fluid was quantified. The data are representative of two independent experiments using 4 mice per group. The errors bars indicate the standard error of the mean between biological replicates.