Loss of hScrib enhances cell invasion.

<p>Panel A. The cell lines were incubated in the upper compartment of matrigel chambers in serum-free medium. After 22 h, the cells that had migrated into the lower serum-containing compartment were fixed and stained with Crystal Violet. The panels show the staining of the cells on the lower surface of the collagen matrix, with the TR2 control cell line and two examples of hDlg1 depleted cells and two examples of the hScrib-depleted cells shown. Panel B shows the quantifications and standard deviations of the numbers of invading cells obtained from at least three independent assays with at least three different control, hScrib and hDlg1 knockdown cell lines.