Knockdown of <i>RBPJ</i> inhibits the growth of rhabdomyosarcoma in nude mice. A,

<p>After transfection of control shRNA or <i>RBPJ</i> shRNA, 1×10⁶ RD cells were subcutaneously inoculated in nude mice (n = 7). Tumor size was calculated weekly by using the formula LW²/2 (with L and W representing the length and width of tumors). <b>B</b>, Survival rate of the mice injected with control shRNA- or <i>RBPJ</i> shRNA-transfected RD cells was assessed by Kaplan–Meier analysis. <b>C</b>, The number of Ki67-positive cells in control shRNA- or RBPJ shRNA-transfected xenograft were accessed by immnohistochemistry. Scale bar is 100 µm. Expression of Cell cycle-related genes (<i>CyclinD</i>, <i>CyclinE</i>, <i>E2F1</i>, <i>SKP2</i>, <i>p21</i>) were assessed by real-time PCR in control shRNA- or RBPJ shRNA-transfected xenograft. The Ct values of xenograft samples were normalized to those of <i>ACTB</i>. The values of the RBPJ shRNA-transfected xenograft was compared with those of the control shRNA sample, which is defined as a relative expression of 1.0. Columns, mean values of 3 independent experiments. Bar, SD. *<i>p</i><0.05, **<i>p</i><0.01.