Flagellate <i>L. ruminis</i> cells or <i>Lactobacillus</i> flagellin stimulate IL8 production by intestinal epithelial cells in a TLR5-dependent manner.

<p>A: Flagellate <i>L. ruminis</i> ATCC27782 cells induce significant IL8 secretion from human intestinal epithelial cell lines. T84, n = 9; HT-29, n = 7; Caco-2, n = 6. The increments on the Y-axis follow a square-root scale. B: Following transfection with siRNA directed against TLR5, IL8 secretion from HT-29 cells was significantly lower in response to ATCC27782 whole bacteria. Boxplots are based on six experimental replicates. Box-plots show the median and the interquartile range. Solid black dots represent outlier values. Statistical significance was determined using a one-tailed Mann-Whitney U test. Horizontal black bars indicate significant differences between samples. The level of statistical significance is given by P-values over these bars, or by the following symbols: P<0.001 = ***; P<0.05 = *; P<0.1 = ▪.