<p>Relative nucleotidase activities expressed as percentage of available substrate dephosphorylated, and is normalized to reactions without inhibitors. The experiment was performed in triplicate using isolated microparticles from three healthy controls and three IPAH patients. *Significant difference from the sample without inhibitor (<i>P</i><0.05).</p
<p>The phosphatase activity was performed in presence of reaction mixture contained 50 µg/ml of wash...
<p>(A) The same amount of purified HC-Pro (60 μg) was added to various amounts of purified NtMinD (i...
<p>The inhibited enzyme activities were assayed with NAD<sup>+</sup> (<b>A</b>) or NADP<sup>+</sup> ...
<p>Km and Vmax values are shown (<b>C</b>). Non-linear regression was used to fit experimental data ...
<p>(<b>A</b>) TLC performed following anti-CD39 pre-treatment of microparticles from healthy control...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ATP...
<p>NT<i>Hi</i> Nuc was incubated with 2 μM FRET substrate and increasing concentrations of inhibitor...
<p>Purified rLicNTPDase-2 was assayed in the absence of inhibitors (control column) or in the presen...
[a]<p>The measurements were performed using 1 mM L-aspartate, 3 µM ADC, and 1 mM compound (potential...
<p>A: Representative example of respiration recording before and after NKA inhibition. In the plot, ...
<p>Each experiment was repeated three times.</p><p>Effects of inhibitors on enzyme activity (%).</p
Nucleotide pyrophosphatase/phosphodiesterase type 1 (NPP1) is a membrane glycoprotein involved in th...
<p>The maximum enzymatic reaction rates (<i>V<sub>max</sub></i> values) calculated for assays perfor...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ADP...
<p>(A) Stimulation of ATP hydrolysis by verapamil (50 µM), QZ59-<i>SSS</i> (1 µM), valinomycin (10 µ...
<p>The phosphatase activity was performed in presence of reaction mixture contained 50 µg/ml of wash...
<p>(A) The same amount of purified HC-Pro (60 μg) was added to various amounts of purified NtMinD (i...
<p>The inhibited enzyme activities were assayed with NAD<sup>+</sup> (<b>A</b>) or NADP<sup>+</sup> ...
<p>Km and Vmax values are shown (<b>C</b>). Non-linear regression was used to fit experimental data ...
<p>(<b>A</b>) TLC performed following anti-CD39 pre-treatment of microparticles from healthy control...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ATP...
<p>NT<i>Hi</i> Nuc was incubated with 2 μM FRET substrate and increasing concentrations of inhibitor...
<p>Purified rLicNTPDase-2 was assayed in the absence of inhibitors (control column) or in the presen...
[a]<p>The measurements were performed using 1 mM L-aspartate, 3 µM ADC, and 1 mM compound (potential...
<p>A: Representative example of respiration recording before and after NKA inhibition. In the plot, ...
<p>Each experiment was repeated three times.</p><p>Effects of inhibitors on enzyme activity (%).</p
Nucleotide pyrophosphatase/phosphodiesterase type 1 (NPP1) is a membrane glycoprotein involved in th...
<p>The maximum enzymatic reaction rates (<i>V<sub>max</sub></i> values) calculated for assays perfor...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ADP...
<p>(A) Stimulation of ATP hydrolysis by verapamil (50 µM), QZ59-<i>SSS</i> (1 µM), valinomycin (10 µ...
<p>The phosphatase activity was performed in presence of reaction mixture contained 50 µg/ml of wash...
<p>(A) The same amount of purified HC-Pro (60 μg) was added to various amounts of purified NtMinD (i...
<p>The inhibited enzyme activities were assayed with NAD<sup>+</sup> (<b>A</b>) or NADP<sup>+</sup> ...