Correlation between <i>rpoS</i> and either P<i>_int</i> or P<i>_inR</i> expression in <i>P. knackmussii</i> B13.

<p>(A) Scatter plots of scaled single cell mCherry fluorescence expressed from P<i>_rpoS</i> and eGFP from P<i>_int</i> (left panel) or P<i>_inR</i> (right panel) in cultures on 3CBA in exponential phase (grey circles) or after 24 h in stationary phase (red-brown circles). (B) As A but showing single cell fluorescence of an RpoS-mCherry fusion protein (under transcriptional control from P<i>_rpoS</i>) versus eGFP fluorescence from P<i>_int</i> (left panel) or P<i>_inR</i> (right panel). Note that in strain B13-3564 and B13-3555 the native <i>rpoS</i> gene is replaced by <i>rpoS-mcherry</i>. Every circle represents measurements on a single cell. The total number of measured cells is displayed in every diagram. (C) Proportion of cells expressing eGFP above threshold (dotted lines in panel B) from P<i>_int</i> (left panel) or P<i>_inR</i> (right panel) in data sets of panel B per quadrant (<i>Q</i>) of normal distributed RpoS-mCherry intensity. <i>Q_1st</i>, from minimum to Q₁ (mean−1 <i>SD</i>); <i>Q_2nd</i>, from Q₁ to Q₂ (mean); <i>Q_3d</i>, from Q₂ to Q₃ (mean+1 <i>SD</i>); <i>Q_4th</i>, from Q₃ to maximum. Letters above bar diagrams indicate significance of difference (P<0.05) in a Tukey's post-hoc test on sample variances (one-way ANOVA).