Prism JD1849 mice have abnormal behavior and Snap25 overexpression.

<p>A) Weights of adult mice are no different from age, sex and strain matched controls. Performance on rotarod is extremely poor: B) JD1849 mice fall or jump off the rotarod more often than matched controls, before apparatus is even turned on. (<i>p<3.5E-5, paired T-Test, n = 8 per group.</i> C) Once habituated enough to stay on apparatus, JD1849 mice still perform significantly worse than matched controls (<i>p<7.5E-05</i>). D) Western blots with antibodies to Aldh1L1, Myc, Mobp, HA, Snap25, and mCherry across development. <i>Actin is shown as a control for equal loading of the pairs within each time point. Total amount of protein loaded in each lane was equal across all time points.</i> E) Western blot for PSD95, shows no difference between wild-type and transgenic mice. F) qRT-PCR on RNA from brains of JD1849 mice shows excess Snap25 mRNA relative to littermate controls across time (<i>p<.04, Paired T-test</i>, <i>two-tailed, n = 10</i>). Aldh1L1 and Mobp were not significantly increased. <i>ddCT method, normalized to Actb. Error bars standard deviation of technical replicates.</i> G) Genotyping of cloned PCR products from brain cDNA and genomic DNA of F1 crosses to strain KK/HIJ. There is an over-representation of the G allele in cDNA (<i>Chi-square test, p<1.2E-5)</i>, suggesting excess Snap25 RNA may be coming from the BAC. H) Lack of apparent overexpression of Snap25 RNA in a Snap25 bacTRAP mouse brain from line JD362. <i>Error bars standard deviation of technical replicates.</i> I) qRT-PCR reveals no significant overexpression of either Snap25 or Mobp RNA in a line double transgenic for Snap25 and Mobp (<i>JD1861),</i> compared to littermates (<i>p>.05, T-test, one-tailed, n = 3 tg, 4 wt). Error bars standard deviation of independent biological replicates.</i>