Modulation of macrophage functions by soluble BAT3.

<p>A. J774A.1 cells were first stimulated with IFN-γ (20 ng/ml) for 2 hours and then purified recombinant BAT3 was added into culture at different concentrations. Total nitrite levels in cell culture supernatants were determined after 18 hours using a colorimetric assay kit. **P<0.01 and ***P<0.001 as compared with IFN-γ only group. B. J774A.1 cells were first incubated with LPS (100 ng/ml) for 2 hours and then stimulated with BAT3 at different concentrations. The levels of IL-1β in cell culture supernatants were determined after 24 hours using ELISA kit from eBioscience Inc. **P<0.01 and ***P<0.001 as compared with LPS only group. C. J774A.1 cells were incubated with LPS (100 ng/ml) for 2 hours and then stimulated with BAT3 at different concentrations. The levels of IL-12p70 in cell culture supernatants were determined after 24 hours using ELISA kit from eBioscience Inc. ***P<0.001 as compared with LPS only group.