VEGFR2-β3 integrin-neuropilin-1 interact <i>in vivo</i> and in cultured podocytes. VEGF knockdown decreases alpha<sub>V</sub>beta<sub>3</sub> integrin activity.
- Delma Veron (151378)
- Guillermo Villegas (151382)
- Pardeep Kumar Aggarwal (151386)
- Claudia Bertuccio (151389)
- Juan Jimenez (151392)
- Heino Velazquez (151396)
- Kimberly Reidy (151400)
- Dale R. Abrahamson (151405)
- Gilbert Moeckel (151410)
- Michael Kashgarian (151414)
- Alda Tufro (151417)
DOIAbstract
<p>(A) VEGFR2 - beta<sub>3</sub> integrin - neuropilin1 (NRP1) co-immunoprecipitate <i>in vivo,</i> shown by reciprocal VEGFR2 and beta<sub>3</sub> integrin IP. Negative control is rabbit serum (RS). Immunoprecipitates were blotted with VEGFR2, beta<sub>3</sub> integrin, Y<sup>1175</sup>-VEGFR2, anti S<sup>785</sup>- beta<sub>3</sub> integrin and neuropilin-1 antibodies. (B) VEGFR2 - beta<sub>3</sub> integrin – NRP1 co-immunoprecipitate in cultured podocytes. IPs were performed as described in (A) using VEGF<sup>KD</sup> podocyte lysates. (C) Dual-immunostaining shows decreased active alpha<sub>v</sub>beta<sub>3</sub> integrin (WOW-1) and total beta<sub>3</sub> integrin in glomeruli from VEGF knockdown mice. (D) Immunocytochemistry shows de...
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