The effects of <i>Pthlh</i> siRNA injection on the expression of <i>Pthlh</i>, PTHLH and <i>Pth1r</i> in mouse preimplantation embryos.

<p>A) Quantitative real-time PCR was applied to detect the expression of <i>Pthlh</i> in the <i>Pthlh</i> siRNA-injected ICSI embryos at the 2-cell stage. Transcripts levels were normalized against <i>Gapdh</i> expression. Data are presented as means ± SEM. *p<0.01. B) <i>Pthlh</i> expression level in 2-cell embryos 24 h post-injection. Data are presented as means ± SEM. *p<0.01. C) <i>Pth1r</i> expression level in 2-cell embryos 24 h post-injection. Data are presented as means ± SEM. *p<0.01. D) Western blot results of PTHLH in the <i>Pthlh</i> siRNA-injected group and the control groups 24 h post-injection, GAPDH was used as the loading control.