Interactive effects of hypoxia and NO drugs on eNOS and CYP1A expression.

<p>Effects of 4 weeks hypoxia (dissolved oxygen, DO: 1.7 mg/L) exposure and pharmacological treatments with <i>S</i>-nitroso-N-acetyl-DL-penicillamine (SNAP, a donor of nitric oxide) and <i>N_ω</i>-nitro-L-arginine methyl ester (NAME, an inhibitor of nitric oxide synthase) on endothelial nitric oxide synthase (eNOS) protein expression (<b>A</b>) and eNOS protein levels (<b>B</b>), CYP1A mRNA levels (<b>C</b>), CYP1A protein expression (<b>D</b>), CYP1A protein levels (<b>E</b>), and ethoxyresorufin <i>O</i>-dethylase (EROD) activity (<b>F</b>) in croaker livers. Each value represents the mean±S.E.M (N = 8–9, tissues were randomly sampled from individual fish for measurements). Significant differences identified with a multiple range test, Fisher’s PLSD, are indicated with different letters (p<0.05). Note: exposure duration only refers to period fish were exposed to target DO; fish were previously exposed to declining DO for additional 2-day adjustment period. CTL, control; HYP, hypoxia; SAL, saline.