Interactive effects of hypoxia and an antioxidant on CYP1A expression.

<p>Effects of 4 weeks hypoxia (dissolved oxygen, DO: 1.7 mg/L) exposure and antioxidant (AOX, vitamin E) treatment on CYP1A mRNA levels (<b>A</b>), CYP1A protein expression (<b>B</b>; Note: representative Western blot shown for samples from individual fish), CYP1A protein levels (<b>C</b>), immunohistochemical expression (<b>D</b>) and immunostaining intensity (<b>E</b>) of CYP1A in croaker liver. Each value represents the mean±S.E.M (N = 8–12, tissues were randomly sampled from individual fish for measurements). Significant differences identified with a multiple range test, Fisher’s PLSD, are indicated with different letters (p<0.05). Note: exposure duration only refers to period fish were exposed to target DO; fish were previously exposed to declining DO for additional 2-day adjustment period. CTL, control; HYP, hypoxia; SAL, saline. Scale bar = 400 µm.