Putative Rho compounds inhibit thrombin stimulated RhoA activation in glioblastoma cells.

<p>Compounds were selected from a pocket directed virtual screen and tested on Glioblastoma cells (1321N1). Cells were pre-treated for 1 hr with either DMSO control or 30 µM of compound 157767, 335504, 25740 or 2561 and subsequently stimulated for 15 minutes with either vehicle or 0.5 U/mL thrombin. RhoA activity was assessed via pull-down by rhotekin and total RhoA was determined using an anti-RhoA antibody. Bar graph shows densitometric results from 4 independent experiments. Data are presented as means ± SEM; *P<0.001 vs. vehicle control, one-way ANOVA.