Confocal images of LepRb localization in hypothalamic ARC neurons in naïve young

<p>(<b>NY</b>)<b>, SCI young</b> (<b>SCIY</b>) <b>and naïve aged</b> (<b>NO</b>) <b>mice.</b> Mouse brain coronal sections (50 μM; −0.7 mm to −2.4 Bregma) were immunostained with LepRb (Red), the neuronal marker NeuN (Green) and counterstained using DAPI (Blue). In NY mice, brain regions corresponding to hypothalamic ARC (Row 1, Blue) are positively immunostained with LepRb (Row 1, Red) and NeuN (Row 1, Green). Higher magnification (Row 2), confocal images show LepRb (Row 2, Red) immunoreactivity is contained within subpopulations of NeuN (Row 2, green) positive cells and LepRb localizes to peripheral membrane regions on the soma of NeuN positive cells (Row 2, Merged). SCIY mice (Row 3, Row 4) have substantially reduced LepRb (Row 3, 4, Red) immunoreactivity in hypothalamic ARC neurons compared to NY control. Similarly, NO mice (Row 5, 6) display substantially less LepRb (Row 5, Row 6, Red) immunoreactivity in hypothalamic ARC neurons compared to NY control. Scale Bars = 50 μM (Row 1, 3, 5), 10 μM (Row 2,4,6).