Putative novel wheat miRNAs discovered by Wei et al. [17] identified in chromosome 1AL survey sequences.

1<p>This pre-miRNA had an identical wheat EST match.</p>2<p>pre-miRNA sequence matched a transposable element, but only one copy was found in 1AL.</p>3<p>miR2023 and miR5050 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040859#pone-0040859-t001" target="_blank">Tables 1</a> & <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040859#pone-0040859-t002" target="_blank">2</a>) derive from opposite arms of the same miRNA:miRNA* duplex.</p>4<p>miR2032 is identical to miR5200 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040859#pone-0040859-t001" target="_blank">Table 1</a>).</p>5<p>Mismatches to the conserved miRNA sequence are underlined and in bold.</p>6<p>MFE = Minimum Folding free Energy of predicted hairpin secondary structure.</p>7<p>MFEI = Minimum Folding Energy Index, calculated as described by Yin et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040859#pone.0040859-Yin1" target="_blank">[40]</a>.