Bacterial localization in the gut of S. littoralis larvae with Fluorescent In Situ Hybridization.

<p>Scale bar equals 10 µm. A, Detection of <i>Clostridium</i> sp. In the midgut. The three images shown are TIC image, fluorescent image of universal probe (EUB, red) and of specific probe (SPE, green). B to G are merged images of TIC, EUB and SPE. The bacteria detected only with universal probe are red, and the bacterial with both probes are green. B, a large aggregate of <i>Clostridium</i> sp. deep in the gut lumen. C, Detection of <i>E. mundtii</i>. D, Detection of <i>E. casseliflavus</i>. E, <i>P. acnes</i> in the midgut. F, <i>E. coli</i> detected in the midgut; G, <i>K. pneumonia</i> detected in the midgut. Bacteria detected only by universal probe are highlighted with white arrows; Bacteria stained by sequence-specific probes are pointed by open arrows. Insect tissue is indicated by arrow heads.