Characterization of the infectivity of HPV pseudovirion in naïve nude mice and human ovarian tumor-bearing nude mice.

<p>5–8 weeks old nude mice were injected intraperitoneally with ES2 human ovarian tumor cells (1×10⁶ cells/mouse). One week later, tumor-bearing mice were intraperitoneally injected with wild-type (wt) HPV-16/Luc psV or mutant L2 (mtL2) HPV-16L1mtL2-Luc pseudovirions (20 µg HPV-16 L1 protein/mouse, equivalent to 120 ng of DNA/mouse). Naïve mice infected with wt or mt HPV-16/luc pseudovirions served as controls. Mice were imaged by non-invasive luminescence imaging 1 day after infection. Data shown are representative of 2 experiments performed.