Analysis of P14 CD8 T cells in the context of a <i>rListeria_GP33</i> infection.

<p>P14.WT, P14.IL-12RKO, P14.IFNARKO or P14.DOKO T cells were transferred into B6 mice followed by infection with <i>rListeria_GP33</i>. Analysis of spleen cells at day 5.5 after infection is shown. <b><i>A</i></b>, Relative and total P14 T cell numbers per spleen is shown. <b><i>B</i></b>, Analysis of cell division by BrdU incorporation. After adoptive transfer, recipient mice were treated with 2 mg of BrdU i.p. on day 4. BrdU staining of P14.WT or P14.DOKO T cells gated on CD8⁺ T cells on day 5 in the spleen is shown. Numbers in brackets indicate percentages of BrdU⁺ cells of total P14 T cells. <b><i>C</i></b>, Analysis of KLRG1 and CD127 expression on P14 T cells. Plots are gated on CD8⁺, Thy1.1⁺ (P14) cells. <b><i>D</i></b>, Statistical analysis of T cell differentiation. Percentages of KLRG1 and CD127 positive P14 T cells of total P14 T cells are depicted. <b><i>E</i></b>, Cytolytic activity of P14 T cells was determined five days post infection by a ⁵¹Cr-release assay. Gp33-41 peptide-loaded or adeno peptide-loaded EL-4 cells (control) were used as targets. Equal numbers of purified P14 T cells were used as effectors. Expression of IFN-γ <b><i>(F)</i></b> and TNF-α <b><i>(G)</i></b> of P14 T cells. Percentages of cytokine positive P14 T cells of total P14 T cells, as well as MFI of cytokine expression are indicated. Values are expressed as mean ± SEM (n = 3). *p<0,01. Results are representative of three independent experiments.