Effects of hypoxia preconditioning on CPCs <i>in vitro</i>.

<p>CPCs were cultured under either normoxia (Nx) or hypoxia (Hx) for 3, 6,12, and 24 hours. (A) Flow cytometry analysis of apoptosis in CPCs cultured under either normoxia or hypoxia at different times. (B) Quantitative analysis of apoptotic cells identified by flow cytometry. Data were obtained from three independent experiments and are expressed as mean ± SD. *<i>P</i><0.01 vs. normoxia (Nx) group. (C) Representative Western blots of HIF-1α, CXCR4, and β-actin protein expression in CPCs (top); quantification of HIF-1α and CXCR4 protein levers (normalized to β-actin protein levels ). (D) Representative Western blots of p-Akt, Bcl-2 and β-actin protein expression in CPCs (top); quantification of p-Akt and Bcl-2 protein levers (normalized to β-actin protein levels). (E) Supernatant VEGF and SDF-1α concentrations measured by ELISA in CPCs that underwent hypoxia preconditioning. Values are mean ± SD. n = 3. *<i>P</i><0.01vs.normoxia.