CD10 expression in cultured macrophages.

<p>(A) Flow cytometry demonstrating that SMC-derived maturing macrophages express CD10 with increasing frequency. Percentages reflect portions of maturing macrophages expressing CD10. Corresponding CD10 versus side scatter (SSC) plots with gates are shown on the left. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040139#pone-0040139-t001" target="_blank">Table 1</a> for kinetics of expression. (B) Confocal microscopy images demonstrating CD10 expression on a young, EDTA-recovered nurse macrophage at D3 of IL-2 exposure. Size bar: 20 µm. (C) Expression of CD10 in macrophages and CD3+ cells recovered from primary macrophage cultures, as determined by real-time PCR. CD3+ cells were purified from the nonadherent populations using CD3+ magnetic beads. Macrophages were harvested using accutase. For each donor, the mean values obtained from uncultured CD3+ cells were subtracted from the mean values shown for CD3+ cells at each time point, and similarly, the mean values obtained from uncultured CD14+ cells were subtracted from the mean values obtained for the cultured macrophages at each time point. No values are shown for CD3+ cells for donor 8892 at days 17, 21 and 24, because too few nonadherent cells were recovered to permit CD3+ magnetic bead selection. The average percent difference between replicate reactions (including those for GAPDH) was 0.6% (donor 4342) and 0.7% (donor 8892).