MFAP-1 is a nuclear protein expressed in multiple tissues.

<p>(A) A 2.5 kb 5′ promoter region upstream of the start codon of <i>mfap-1</i> was used to drive the expression of a transcriptional fusion GFP reporter in transgenic animals. The tissues in which the GFP reporter was expressed are labeled. This figure shows a montage of two photographs, which were pseudo-colored from a gray-scale image to a green-scale image using Adobe Photoshop to reflect the level of GFP expression. (B) Transgene experiments were performed as described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002827#s4" target="_blank">Materials and Methods</a>. The <i>C. elegans</i> MFAP-1::GFP fusion protein, when expressed in body-wall muscles using a <i>myo-3</i> promoter in either wild-type or <i>mfap-1(n4564 n5214)</i> animals of mixed developmental stages, was exclusively localized in nuclei (left and middle panels). Similarly, human MFAP-1::GFP fusion protein, when expressed in the body-wall muscles of <i>mfap-1(n4564 n5214)</i> animals, was also exclusively localized in nuclei (right panels). Lower panels show typical nuclear GFP signals in adult transgenic animals. Genetic backgrounds and transgenes are indicated.