Mitochondrial functional parameters: differential effects of HIV-1 infection and ART on lymphocytes and monocytes.

<p>(<b>A, E</b>) Discriminant analysis using ROS and ΔΨm parameters. The five cohort populations are delineated by their 95% confidence circles around the means. The perpendicular axes describe the combined variance of the parameters analysed (lymphocytes: 77.26%, monocytes: 92.25%). The contributions of each parameter to the variances on the X and Y axes are shown in the insets. (<b>A</b>) Lymphocytes. ART naive patients are statistically different to control subjects. ART partially rescues mitochondrial parameter changes induced by HIV infection. Inset: The 8 mitochondrial parameters used were: a: Basal ROS production by high-ROS lymphocytes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041129#pone-0041129-g003" target="_blank">Figure 3B</a>). b: PMA/Basal ratio of ROS production by high-ROS lymphocytes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041129#pone-0041129-g003" target="_blank">Figure 3C</a>). c^High: ΔΨm of high-ΔΨm lymphocytes. c^Low: ΔΨm of low-ΔΨm lymphocytes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041129#pone-0041129-g003" target="_blank">Figure 3D</a>). c^Low%: Percentage of low-ΔΨm lymphocytes. d^High: Basal to CCCP ΔΨm ratio of high-ΔΨm lymphocytes. d^Low: Basal to CCCP ΔΨm ratio of low-ΔΨm lymphocytes. d^Low%: CCCP to Basal ratio of low-ΔΨm lymphocyte percentage. (<b>E</b>) Monocytes. ART naive patients are close to control subjects whereas ART combinations are more dispersed. Inset: The 4 mitochondrial parameters used were: a: Basal ROS production by high-ROS monocytes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041129#pone-0041129-g003" target="_blank">Figure 3F</a>). b: PMA to Basal ratio of ROS production by high-ROS monocytes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041129#pone-0041129-g003" target="_blank">Figure 3G</a>). c: ΔΨm of monocytes (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041129#pone-0041129-g003" target="_blank">Figure 3H</a>). d: Basal to CCCP ΔΨm ratio of monocytes. (<b>B to D; F to H</b>) Box plots (XLSTAT) displaying 1st quartile (Q1), median, mean (displayed by ‘+’), 3rd quartile (Q3) together with both lower and upper limits were calculated as follows: lower limit = X_i such that {X_i – [Q1–1.5 (Q3– Q1)]} is the minimum and X_i ≥ Q1–1.5 (Q3– Q1); upper limit = Y_i such that { Y_i – [Q3+1.5 (Q3– Q1)]} is the minimum and Y_i ≤ Q3+1.5 (Q3– Q1). *p<0,05, **p<0,01.