CD14⁺ monocytes reconstitute apoptosis in CD3⁺ T-cells following Streptococcus pneumoniae challenge.

<p>A) Peripheral blood mononuclear cells (PBMC), purified CD3⁺ T-cells (CD3⁺ CD14⁻) or co-cultures of purified CD3⁺ T-cells with 10% purified CD14⁺ monocytes (CD3+ CD14+) were mock-infected (MOI = 0, white bars) or challenged with D39 <i>Streptococcus pneumoniae</i> (MOI = 50, black bars) for 16 h and accumulation of hypodiploid DNA (% Sub G0/1) measured in CD3⁺ T-cells, n = 4. B) caspase 3 activation in CD3⁺ T-cells under the same condition as A), n = 4. C) Representative western blot probed for Bid, Bim and actin from CD3⁺ T-cells isolated from PBMC or CD3 enriched cultures following bacterial challenge as in A), D) densitometry summarizes the fold change in Bid compared to the mock infected (MI), derived from three separate experiments. E) % Sub G0/1 purified CD3⁺ T-cells in co-cultures containing 10% or 20% of purified CD14⁺ monocytes under the same conditions as A), n = 4 or in F) co-cultures of CD3⁺ T-cells and purified CD14⁺ monocytes with (CD14⁺/CD16⁻) or without (CD14⁺/CD16⁺) CD16⁺ monocyte depletion, under the same conditions as A), n = 4, ns (not significant) * p<0.05, ** p<0.01, *** p<0.001; statistical analysis by ANOVA.