Growth characteristics of North American TRS viruses in MDCK cells.

<p>Cells were inoculated with the respective viruses at an MOI of 0.001 and incubated at the indicated temperatures. At the indicated h p.i., supernatants were harvested and virus was titrated by pfu assay. At the higher temperatures (37°C and 39.5°C), cytopathic effects caused detachment of most infected cells from the dish after 38 h p.i.; therefore, virus release was not examined beyond that point. Values are the mean of two independent experiments performed in duplicate (n = 4).