Expression of Rab GTPase genes on primary macrophages after infection with <i>E</i>. <i>coli</i>.

<p>Columns represent the relative quantification of the mRNA levels of each Rab GTPase, analyzed by real-time quantitative PCR, after reverse transcription of total mRNA and normalized against GAPDH gene expression levels. Macrophages were incubated with live or dead (HK) bacteria at a multiplicity of infection (MOI) of 30∶1 (<i>E</i>. <i>coli</i> 30, <i>E</i>. <i>coli</i> 30 HK) and 10∶1 (<i>E</i>. <i>coli</i> 10, <i>E</i>. <i>coli</i> 10 HK). Cultures of macrophages with medium only (Medium) and cultures with LPS (1 µg/ml) or latex beads (beads) were also performed. Error bars indicate the standard error of the mean of 5 independent experiments. Statistical significance (**p<0.01, ***p<0.001) refers to the difference between macrophages incubated with live or dead bacteria or incubated with LPS or latex beads and macrophages incubated with medium alone.