Z<sub>HER</sub>2 binding affinity.
- Rafal Zielinski (150261)
- Moinuddin Hassan (150266)
- Ilya Lyakhov (150272)
- Danielle Needle (150280)
- Victor Chernomordik (150287)
- Alejandra Garcia-Glaessner (150295)
- Yasaman Ardeshirpour (150303)
- Jacek Capala (150309)
- Amir Gandjbakhche (150312)
Publication date
July 2012
DOIAbstract
<p>A. BT-474 cells were incubated for one hour with increasing concentration of Z<sub>HER2</sub>-GSC-DyLight-488 or Z<sub>Taq</sub>-GSC-DyLight-488. B. BT-474 cells were pre-incubated with increasing concentration of non-modified Z<sub>HER2</sub> molecules with or without flexible spacer Z(HER2-GS-Cys and Z<sub>HER2</sub>-Cys respectively), followed by addition of 0.5 µg/ml of Z<sub>HER2</sub>-DyLight-48. Fluorescence intensity was measured in triplicates using LSR II flow cytometer. FlowJo (Tree Star Inc, Ashland, OR) was used for FACS data analysis. K<sub>D</sub> and IC50 values were calculated using GraphPad Prism (GraphPad Software, Inc., San Diego, CA).</p
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