Dicer function during endometrial decidualization.

<p><b>A</b>, A representative Western blot of DICER protein levels in control siRNA decidualized hESCs (Control siRNA (cAMP+MPA)) and Dicer knockdown decidualized hESCs (Dicer siRNA (cAMP+MPA)) at 48 h and 72 h after decidual treatment. <b>B</b>, Prolactin, IGFBP-1 and MPIF-1 secretion levels in the non decidualized control hESCs, the control siRNA decidualized hESCs (Control siRNA (cAMP+MPA)) and the Dicer knockdown decidualized hESCs (Dicer siRNA (cAMP+MPA)) measured by ELISA at 48 h and 72 h after decidual treatment. Prolactin, IGFBP-1 and MPIF-1 secretion levels were normalized to the total amount of protein present in the media. Data represent the mean of four independent experiments. Error bars represent the SEM. Statistical analysis, * p<0.05. <b>C</b>, TIMP-3 secretion levels measured by Western blot in the control siRNA decidualized hESCs (Control siRNA (cAMP+MPA)) and the Dicer knockdown decidualized hESCs (Dicer siRNA (cAMP+MPA)) at 72 h after decidual treatment. Ponceau staining of membranes was used as a protein-loading control. Only the 60 kDa band of the ponceau gel is shown. Data represent the mean of four independent experiments. Error bars represent the SEM.