The role of LEDGF in LPA induced VEGF-C expression.

<p>PC-3 cells were treated with LPA and mRNA (A) and protein (B) expression levels of LEDGF were determined. LEDGF proteinm was collected after 2 hr LPA treatment. Pretreatment with 10 µM Ki16425 and 10 mM NAC for 1 h suppressed the effects of LPA (L5: 5 µM LPA) on LEDGF expression (C and D). LEDGF siRNA was transfected into PC-3 cells (E) and suppressed LPA’s effects on VEGF-C expression (F). mRNA was reverse-transcribed and analyzed by a real-time PCR. The relative levels of LEDGF and VEGF-C normalized to GAPDH are shown. * Statistically different compared to vehicle-incubated samples treated with LPA (*<i>p</i><0.05; **<i>p</i><0.01; ***<i>p</i><0.001).