Cloning of flagellum gene cluster and glycosylation island and construction of <i>fliC</i> and <i>fgt1</i> mutants.

<p>(A) Organization of partial flagellum gene cluster and glycosylation island. The black lines represent the PCR fragments amplified by indicated primer pairs (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041056#pone.0041056.s003" target="_blank">Table S1</a>) as shown with arrows above the fragments. The constructs used to delete <i>fliC</i> and <i>fgt1</i> genes are aligned below the gene structure. (B) <i>fliC</i> mutant was screened by PCR using primers PC1 and PD2 as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041056#pone-0041056-g001" target="_blank">Figure 1A</a>. (C) <i>fgt1</i> mutant was screened by PCR using primers prLTail and prOR1–2 as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041056#pone-0041056-g001" target="_blank">Figure 1A</a>.