Overview of the CRISPR/Cas systems in pNG400 and <i>Natrinema</i> sp. J7-2.

<p>(A) The Hmari CRISPR5/Cas system in pNG400. (B) The J7 CRISPR1/Cas system in <i>Natrinema</i> sp. J7-2. The Cas genes (<i>blue</i>), CRISPRs (<i>purple</i>), integrase gene and IS6 family transposase gene (<i>yellow</i>) are drawn to scale as arrows. The accession numbers of the Cas genes are shown above the arrows. Diamonds and rectangles represent the direct repeats and spacers, respectively. Direct repeats with identical sequences are shown in the same color. The sequences of the direct repeats are given, and the nucleotides that differ from those of the direct repeat of J7 CRISPR1 are shown in red. The GC content is plotted above the diagrams of the CRISPR/Cas regions, with the average GC content for the region indicated as a straight black line (average % GC is given at the right).