Alteration of insulin-like growth factor signaling pathway gene functions.

<p>Expression of the PSC cell-specific markers <i>hhF4f-GFP</i> and Antp was assessed in lymph glands of the following loss- or gain-of-function genotypes: (A) wild-type, (B) <i>col>dAkt1</i> RNAi, (C) <i>col>dAkt1</i> cDNA, (D) <i>col>PDK1</i> RNAi, (E) <i>col>InR^DN</i> cDNA, (F) <i>col>InR</i> cDNA, (G) <i>col>Pi3K92E</i> RNAi, (H) <i>col>Pi3K92E^DN</i> cDNA, and (I) <i>col>Pi3K92E^CA</i> cDNA. Arrows in panels C, F, and I point out expanded populations of PSC niche cells, with an abnormal niche organization caused by PSC cell-specific expression of the <i>InR</i> cDNA. Arrowheads in panels B, D, E, G, and H point out reduced populations of PSC niche cells. Scale bar indicates 20 µm.