Alteration of <i>Pten</i> and <i>dFOXO</i> gene functions.

<p>Expression of the PSC cell-specific markers <i>hhF4f-GFP</i> and Antp was assessed in lymph glands of the following loss- or gain-of-function genotypes: (A) wild-type, (B) <i>Pten¹⁰⁰/Pten¹¹⁷</i>, (C) <i>P85col>Pten</i> cDNA, (G) <i>dFOXO²¹/dFOXO²⁵</i>, (H) <i>col>dFOXO</i> cDNA, and (I) <i>Pten¹¹⁷/+;dFOXO²¹/+</i>. Arrows in panels B, G, and I point out expanded populations and abnormal organization of niche cells. Arrowheads in panels C and H point out severely reduced populations of PSC niche cells. (D) Wild-type lymph gland assayed for DAPI (DNA), P1 antigen (plasmatocytes), and <i>dome-lacZ</i> expression (hematopoietic progenitors). (E) <i>Pten¹⁰⁰/Pten¹¹⁷</i> lymph gland assayed for DAPI, P1 antigen, and <i>dome-lacZ</i> expression. The arrow points out the substantial increase in plasmatocyte number. (F) <i>Pten¹⁰⁰/Pten¹¹⁷</i> lymph gland assayed for <i>MSNF9mCherry</i> transgene activity (lamellocytes). The arrow points out the de novo production of lamellocytes. Scale bar indicates 20 µm.