Alteration of TOR signaling pathway gene functions.

<p>Expression of the PSC cell-specific markers <i>hhF4f-GFP</i> and Antp was assessed in lymph glands of the following loss-of-function genotypes: (A) <i>col>Tor</i> RNAi, (B) <i>col>raptor</i> RNAi, (C) <i>col>rictor</i> RNAi, (D) <i>col>TSC1</i> RNAi, (G) <i>col>TSC2</i> RNAi, (J) <i>S6K^L-1/S6K^L-1</i> (Antp only), and (K) <i>Thor⁰⁶²⁷⁰/Thor⁰⁶²⁷⁰</i>. Arrows in panels G and K point out expanded populations and abnormal organization of PSC niche cells. Arrowheads in panels A, B, C, and J point out severely reduced populations of PSC niche cells. (E) Expanded expression of the <i>hhF4f-GFP</i> transgene in <i>TSC1^f01910/TSC1^f01910</i> lymph glands. (F) Increase in plasmatocyte number (detected by P1 antibody) and supernumerary lamellocyte production (detected by <i>MSNF9mCherry</i> activity) in <i>TSC1^f01910/TSC1^f01910</i> lymph glands. (H) Expanded expression of the <i>hhF4f-GFP</i> transgene in <i>TSC2¹⁰⁹/TSC2¹⁰⁹</i> lymph glands. (I) Increase in plasmatocyte number and supernumerary lamellocyte production in <i>TSC2¹⁰⁹/TSC2¹⁰⁹</i> lymph glands. Scale bar indicates 20 µm.