Discriminant analysis of molecular species profiles according to lipid class and membrane fraction.

<p>PE, PC, PI and PG profiles were analyzed by MRM mass spectrometry in different membrane fractions. The number of repetitions <i>n</i> for each phospholipid class and for each membrane fraction is indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041985#pone.0041985.s006" target="_blank">Figure S6G</a>. The profiles were used for a discriminant analysis. The score plot represents 86% of the total variability of molecular species profiles. F1/F2 are the 2 principal eigenvalues for this variability. The Variables (phospholipid molecular species)/Factors (F1 and F2) correlations are shown in the loading plot (insert). The score for each profile was calculated. For clarity, only the centroid corresponding to one phospholipid class in a specific membrane is shown. Each class associates a specific phospholipid (PC, PE, PI, PG) with the extract prepared from a sub-cellular membrane fraction. The full image where all scores are presented is in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041985#pone.0041985.s006" target="_blank">Figure S6E</a>. Abbreviations: mit,mitochondria; n, nucleus; g, Golgi apparatus; m, microsomes; h, homogenate; d, DRM; r, RE; to, tonoplast; p, plasma membrane.