Expression of wild type and mutant HAs in the baculovirus system.
- Lyubov Popova (149073)
- Kenneth Smith (149075)
- Ann H. West (149078)
- Patrick C. Wilson (149079)
- Judith A. James (149082)
- Linda F. Thompson (149086)
- Gillian M. Air (149091)
Publication date
July 2012
DOIAbstract
<p>1. Wild type 309 HA 2. Mutant HL156-157KS 3. Mutant KFK158-160GST 4. NDQI189-192QEQT 5. A196V 6. N133D 7. TSSS135-138GSNA 8. K140I. Supernatant (25 µl) from a 25 cm<sup>2</sup> flask of Sf9 cells infected with the recombinant baculoviruses expressing wild type and mutant HA was loaded on a 12% SDS-polyacrylamide gel. HA was visualized by immunoblotting assay with anti-HA tag (YPYDVPDYA) polyclonal antiserum.</p
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